Flow cytometric analyses with monoclonal antibodies were so
far mainly restricted to cell surface molecules. Intracellular
structures such as cytoplasmic or nuclear enzymes, oncoproteins,
cytokines, immunoglobulins etc. were largely excluded from such
studies.
Also excluded from flow cytometric studies were cytoplasmic
localizations of well established membrane molecules like CD3
and CD22, which, in their cytoplasmic form, are the most reliable
lineage markers in undifferentiated leukemia. With the
FIX&PERM® Kit flow cytometric analysis of
intracellular antigens has become as easy as surface antigen
studies.
| |
|
Cat.No.
|
Quantity |
Tests |
Datasheet |
Antibodies
optimized for use with FIX&PERM® |
| RUO |
FIX&PERM®
Kit |
GAS-002 |
2 x 20ml |
200 Tests |
|
|
| RUO |
FIX&PERM®
Sample Kit |
GAS-002M |
2 x 5ml |
50 Tests |
|
|
| RUO |
FIX&PERM®
Kit 1000 |
GAS-002-1 |
2 x 100ml |
1000 Tests |
|
|
| RUO |
FIX&PERM®
Solution A (Fix) |
GAS-002A-1 |
1 x 100ml |
1000 Tests |
|
|
| RUO |
FIX&PERM®
Solution B (Perm) |
GAS-002B-1 |
1 x 100ml |
1000 Tests |
|
|
The FIX&PERM® Cell Permeabilization Kit contains
2 reagents: Fixation Medium (Reagent A) and Permeabilization
Medium (Reagent B). It is intended for first fixing cells in
suspension with Reagent A and then permeabilizing the cell membranes
with Reagent B. This procedure gives antibodies access to intracellular
structures and leaves the morphological scatter characteristics
of cells intact.
Specific formulations reduce background staining and allow simultaneous
addition of permeabilization medium and fluorochrome labeled
antibodies. FIX&PERM® is suitable for the
analysis of normal and malignant leukocyte populations derived
from various human biological samples (blood, bone marrow and
others) using flow cytometry. |
 |
 |
|
